Part 1  Basic techniques of biochemistry and molecular biology
  Chapter 1  Basic techniques of preparation of biomacromolecules
    Section 1  Salting out
    Section 2  Dialysis and ultrafiltration
  Chapter 2  Spectrophotometry
    Section 1  Basic principles
    Section 2  The basic construction of spectrophotometers
    Section 3  Some home-made spectrophotometers
  Chapter 3  Chromatography
    Section 1  Adsorption chromatography
    Section 2  Partition chromatography
    Section 3  Ion-exchange chromatography
    Section 4  Gel filtration
    Section 5  Affinity chromatography
  Chapter 4  Electrophoretic techniques
    Section 1  Basic principles
    Section 2  Several types of commonly used electrophoresis
  Chapter 5  Centrifugation techniques
    Section 1  Basic principle
    Section 2  General description of centrifugation
  Chapter 6  Genetic engineering and analytieal technology of gene expression
    Section 1  Genetic engineering
    Section 2  Polymerase chain reaction
    Section 3  Nucleic acid hybridization
    Section 4  DNA sequencing technology
Part 2  Experiments of the biochemistry and molecular biology
  Chapter 7  Analysis of purification and physicochemical properties of proteins
    Experiment 1  Colorimetric methods for protein determination
    Experiment 2  Isolation and purification of serum y-globulin
    Experiment 3  Cellulose acetate membrane electrophoresis of serum proteins
    Experiment 4  Determination of relative molecular mass of protein in SDS-PAGE
  Chapter 8  Kinetics of enzyme-catalysed reactions
    Experiment 1 The specificity of enzyme and effect of temperature, pH on the enzyme Activity
    Experiment 2  Isolation of alkaline phosphatase and determination of its specific activity
    Experiment 3  Effects of substrates concentration of enzymatic activity: determination of Km of alkaline phosphatase
    Experiment 4  Inhibition effect of phosphate on alkaline phosphatase
    Experiment 5  Analysis of lactic dehydrogenase isoenzyme
  Chapter 9  Substance metabolism
    Experiment 1  Extraction and identification of liver glycogen
    Experiment 2  Determination of alanine transaminase activity (Reitman-Frankels'method)
    Experiment 3  Enzyme catalyzed transamination of amino acids detected by paper chromatography
  Chapter 10  Basic experiment of molecular biology
    Experiment 1  Preparation of the genomic DNA in the eukaryotic cells
    ExPeriment 2  Determination of the concentration and purity of DNA by spectroscopy
    Experiment 3  Agarose gel electrophoresis of nucleic acids
    Experiment 4  Polymerase chain reaction (PCR)
    Experiment 5  Isolation and purification of the DNA fragments
    Experiment 6  DNA ligation--constructing recombinant plasmids
    Experiment 7  Preparation of li competent cells
    Experiment 8  Transformation of Ca2+ competent ceils
    Experiment 9  The screening of recombinant DNA
    Experiment 10  Minipreparation of plasmid DNA
    Experiment 11  Digestion of plasmid DNA with restriction endonucleases
    Experiment 12  Southern blot
    Experiment 13  Expression of exogenous gene in prokaryotic cells
References
Appendix
  Appendix I  The calculation table of ammonium sulfate saturation
  Appendix II  Common buffer solution preparation
  Appendix III  The relative molecular mass and pI of protein
  Appendix IV  Common data of chromatography
  Appendix V  The conversion between centrifugation speed of centrifugation force